Elucidating mechanism cellular uptake


01-May-2019 17:34

elucidating mechanism cellular uptake-11

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The ideal gene delivery and transfection systems should have high transfection efficiency, low toxicity to the cells and single cell specificity, while also is able to simultaneously treat heterogeneous systems with many different cells. Current gene transfection systems contain three major groups: viral (transduction), physical (direct micro injection) and chemical methods. Table 2 Detailed information and cell types (in nine cell lines) with relatively higher luciferase gene transfection efficiency without fetal calf serum of six commercially available transfection reagents. Although both viral systems and physical methods have drawn much attention of the researchers, they suffer from a number of drawbacks as described above. Enhanced gene delivery mediated by low molecular weight chitosan/DNA complexes: effect of p H and serum. Currently, both gene therapy and nanotechnology are controversial topics which have and will get much skepticism from both the general public and researchers for their significant safety problems. Nevertheless, considerable achievements in clinical use have already been made in both gene therapy and technology through the efforts of researchers [35, 36]. Hydrophobic modifications of cationic polymers for gene delivery.

Currently, a variety of synthetic transfection reagents have been commercialized for in vitro gene transfer and both their detailed information and the comparison results of transfection efficiency are listed in Table 2 [34]. Synthesis and characterization of chitosan-g-poly(ethylene glycol)-folate as a non-viral carrier for tumor-targeted gene delivery. Thiolated trimethyl chitosan nanocomplexes as gene carriers with high in vitro and in vivo transfection efficiency. Synthesis and characterization of amphiphilic glycidol-chitosan-deoxycholic acid nanoparticles as a drug carrier for doxorubicin. To overcome these drawbacks, a variety of chemical transfection systems, such as calcium phosphates, lipids, and cationic polymers including polyamidoamine dendrimers and polyethylenimine (PEI), etc, have been developed since late 1960s [31-32]. Chemical gene delivery systems were recognized as an alternative to viral gene vectors for their potential in avoiding some problems associated with the viral systems [33]. High efficiency gene transfer using chitosan/DNA nanoparticles with specific combinations of molecular weight and degree of deacetylation. How to cite this article: Jin L, Zeng X, Liu M, Deng Y, He N. Available from transfer methods are promising in the field of gene therapy.

Current Progress in Gene Delivery Technology Based on Chemical Methods and Nano-carriers. Current methods for gene transfer include three major groups: viral, physical and chemical methods. Despite increasing understanding of the molecular mechanism of cancers [2, 3], many malignancies remain resistant to established traditional treatments [4, 5].




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