Validating microarray data using rt real time pcr
Therefore, soil monitoring and understanding changes in soil ecosystem are considered to be important aspects of any environmental monitoring.The recent emergence of Wireless Biosensor Networks (WBNs) promises addressing many ecological questions, particularly related to soil, though providing uninterrupted real-time data from various biosensors using RF signals.This cookie stores just a session ID; no other information is captured.Accepting the NEJM cookie is necessary to use the website.In the second part of our experiment, we used regular gardening soil mostly composed of organic matter, perlite and vermiculite.Vermiculite keeps the soil hydrated and is part of the clay minerals group. In both cases, we maintained the temperature at 23 degrees Celsius.On the other hand, the path loss in sand is about 0.9-1.2 d B more than air.It is therefore, preferred to use lower band frequencies for underground biosensors.
The ability of the transformed plants to absorb pollutants will be evaluated by performing gas chromatography mass spectrometry analyses to measure pollutants in the inlet and outlet airflow in order to quantify the pollutant deposition rate.
In this work we focused on understanding the impact of soil composition (e.g., clay content) as RF waves travel underground.
This study is crucial in order to define the appropriate RF band, power level, and RF antenna size for extended underground monitoring.
To achieve this goal we genetically transformed Arabidopsis thaliana with an ACS (Aminocyclopropane-1-carboxylic acid synthase) gene.
Using quantitative real-time PCR (q PCR), we previously confirmed that the ACS gene was upregulated in poplar (Populus trichocarpa) plants when exposed to environmental pollutants including ozone and methyl vinyl ketone.We measured the RF signal path loss through the sand for the above frequencies at different horizontal distances between the transmitter and receiver.